Instrumentation

LTQ Orbitrap ELITE ETD (Thermo fisher)

Orbitrap Elite mass spectrometer with Nanospray Flex ion source and UltiMate 3000 RSLC system
The Orbitrap Elite is a high performance hybrid mass spectrometer combining the high-field OrbitrapTM mass analyzer with the latest dual pressure linear ion trap, allowing increased speed, sensitivity and dynamic range. This instrument achieves routinely ultra-high resolution (>100,000) and reliable mass accuracy. It enables multiple fragmentation techniques such as CID, HCD and ETD.This instrument can perform a wide variety of experiments from in-depth discovery based experiments to qualitative and quantitative experiments.

It is equipped with the heated ESI (H-ESI II) and the nanoESI (nanoSpray Flex Ion) sources, as well as the nanochiped based infusion Advion TriVera Nanomate®. Mass accuracy is typically <3 ppm with external calibration. Finally, LC-MS and LC-MSn experiments can be performed since this Orbitrap Elite is interfaced with a nanoUPLC Ultimate 3000 system (Dionex).

Orbitrap Q Exactive HF (Thermo Fisher)

orbitrap

The Thermo Fisher Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer (abbreviated QE HF)

The Q Exactive HF system combines a state-of-the-art segmented quadrupole for high-performance precursor ion selection with a high-resolution, accurate-mass (HR/AM) ultra-high-field Orbitrap mass analyzer. This instrument achieves routinely high resolution (>100,000 at 400 m/z) and reliable mass accuracy. It enables HCD fragmentation and can perform a wide variety of experiments from in-depth discovery based experiments to qualitative and quantitative experiments. It is equipped with the heated ESI (H-ESI II) and the AP-MALDI source (MassTech Inc.). Mass accuracy is typically <3 ppm with external calibration. Finally, LC-MS and LC-MSn experiments can be performed since this instrument is interfaced with a UPLC Ultimate 3000 system (Dionex).

MALDI-TOF AutoFlex speed (Bruker)

Maldi-tof autoflex The MALDI-TOF AutoFlex speed from Bruker has advanced performances for a broad range of applications, among them Peptide/protein, Glycan and PTM analyses. The instrument is capable of operation in reflector mode and linear mode (up to 100 kDa for intact proteins and large molecules such as polymers), as well as TOF/TOF tandem mass spectrometry. Its SmartbeamTM-II 2 kHtz laser and system electronics allow intensive and high throughput applications. This system has a broadband mass resolution and accuracy (<2 ppm and maximum resolving power >26,000), and a minimized maintenance thanks to its IR laser-based self-cleaning Ion Source. This is an open-access instrument (training required) well suited for analysis of peptides and proteins, as well as small organic components, organometallics, nucleic acids, sugars and polymers. In open-access after a mandatory training provided by the service.

Xevo G2-S QTOF (Waters)

The Xevo® G2-S QTof is equipped with the StepWave™ ion optics to achieve high sensitivity levels and the QuanTof™ technology for high mass resolution, matrix-tolerant high dynamic range, quantitative performance, mass accuracy and speed of analysis. The mass analyzer is interfaced with Acquity UPLCpumps and sample manager systems. UPLC-MS and MS/MS can be acquired using the UPLC/MSE data acquisition or Fast data directed analysis (FastDDA) modes, depending on the project and application. IntelliStart™ guarantees maximum system performance and usability for reproducible results. The instrument is equipped with ESI-APCI sources, as well as the APPI photoionization source.

6530 Accurate-Mass Q-TOF LC/MS (Agilent Technologies)

The 6530 Q-TOF incorporates the Agilent Jet Stream thermal gradient focusing technology, which dramatically improves system sensitivity to high femtogram levels of detection. Its TOF technology delivers exceptional mass accuracy, resolution (20,000 not dependent on spectral acquisition rate) and dynamic range across a wide range of concentrations. It can be operated in a brad mass range mode (m/z 25 to 20,000). The instrument is interfaced with Agilent 1290 Infinite UHPLC modules and equipped with the Dual Jetstream ESI and APPI photoionization sources.

LCQFleet (Thermo fisher)

LCQFleet The LCQFleet Ion Trap mass spectrometer provides high sensitivity MS at fast cycle time measurements, and a fast positive/negative switching ionization mode. Enhanced and zoom scan allow better resolution and reliable charge states recognition, and the Selected Ion Monitoring (SIM) mode is used for target compound analysis. The instrument can be operated in Full-scan MS/MS mode, in Selected Reaction Monitoring (SRM) for quantitative experiments and MSn for multi-stage MS experiments to probe the structure of ions.
The LCQFleet is equipped with the Ion Max-S API source and interfaced with a Dionex Ultimate 3000 UPLC system. This instrument is open-access after a mandatory training provided by the service. Open-access is restricted to EPFL members.

TSQ8000 Triple Quadrupole GC-MS/MS (Thermo fisher)

TSQ8000 The TSQ8000 Triple Quadrupole GC-MS/MS instrument is paired with the Thermo scientific TRACE 1300 GC and the autosampler AI1310. The Triple quadrupole can work in Full scan, SIM, SRM and product ion-scan modes. It offers an extractable EI ion source with a vacuum probe interlock to remove entire source without venting. The mass range us 1.2-1200 m/z, and scan speed up to 11000 u/sec. Electron Energy of the source is adjustable from 0 to 150 eV. Transfer line temperature goes up to 400°C.  Data are processed using XCalibur and TraceFinder Software for GC-MS quantification, as well as the NIST libraries for compound identification.
El causes much fragmentation than Cl: the sample is bombarded with electrons, causing the ejection of one or more electrons according to the following equation:

M + e       →    M+ + 2e

Cl causes less fragmentation than El. The reagent gas is ammonia and the most common ions detected are the protonated species and ammonium ion adducts.

NH3 + e       →          NH4+ + 2e

NH4+ + M     →           NH3 + [M+H]+

NH4+ + M     →           [M+NH4]+

The TRACE 1300 GC is equipped with a Capillary column VF-5ms (5% phenyl-methyl 95% dimethylpolysiloxane column, 0.25mm x 30 m) suitable for a wide range of applications: alcohols, amines, aromatic hydrocarbons, drugs, esters, flavors, glycerides, halogenated compounds, hydrocarbons, organic acids, pesticides, phenols, polymers, steroids, sterols, sugars, sulfur compounds…GC is the method of choice for the separation of volatile and low-mass range components.

ICP-MS (NexIon 350 (Perkin Elmer)

icp-ms The NexION® 350D inductively coupled plasma mass spectrometer features a dual-channel Universal Cell and the ability to use Dynamic Reaction Cell™ technology, giving the flexibility to choose Kinetic Energy Discrimination (KED) and/or use a scanning quadrupole in the Dynamic Reaction Cell.
The instrument provides enhanced sensitivity (down to low ppt level for certain metals) and allows both precise quantitative and semiquantitative (screening mode) analysis of various metals. This makes it ideal tool for inorganic synthesis, biomonitoring, toxicology, and food testing applications.